Phosphorylation of spore coat proteins during development of Dictyostelium discoideum
نویسندگان
چکیده
منابع مشابه
Regulation of protein synthesis during spore germination in Dictyostelium discoideum.
Spore germination in the slime mold Dictyostelium discoideum is a particularly suitable paradigm for studying the regulation of gene expression because developmentally regulated changes in both protein and mRNA synthesis occur during the synchronous transition from dormant spore to growing ameba. To investigate the regulation of protein synthesis during germination, we labeled activated spores ...
متن کاملRole of SP65 in assembly of the Dictyostelium discoideum spore coat.
Like the cyst walls of other protists, the spore coat of Dictyostelium discoideum is formed de novo to protect the enclosed dormant cell from stress. Spore coat assembly is initiated by exocytosis of protein and polysaccharide precursors at the cell surface, followed by the infusion of nascent cellulose fibrils, resulting in an asymmetrical trilaminar sandwich with cellulose filling the middle ...
متن کاملA linking function for the cellulose-binding protein SP85 in the spore coat of Dictyostelium discoideum.
SP85 is a multidomain protein of the Dictyostelium spore coat whose C-terminal region binds cellulose in vitro. To map domains critical for localizing SP85 and for binding to other proteins in vivo, its N- and C-terminal regions, and a hybrid fusion of the N- and C-regions, were expressed in prespore cells. Immunofluorescence showed that only the N-terminal region and the N/C-hybrid accumulated...
متن کاملMultiple O-Glycoforms on the Spore Coat Protein SP96 in Dictyostelium discoideum
A decreased level of fucosylation on certain spore coat proteins of Dictyostelium discoideum alters the permeability of the spore coat. Here the post-translational modifications of a major spore coat protein, SP96, are studied in a wild type strain (X22) and a fucosylationdefective mutant (HU2470). A novel phosphoglycan structure on SP96 of the wild type strain, consisting of Fuc(a1–3)GlcNAc-a-...
متن کاملPhagosomal proteins of Dictyostelium discoideum.
In recognizing food particles. Dictyostelium cell-surface molecules initiate cytoskeletal rearrangements that result in phagosome formation. After feeding D. discoideum cells latex beads, early phagosomes were isolated on sucrose step gradients. Protein analyses of these vesicles showed that they contained glycoproteins and surface-labeled species corresponding to integral plasma membrane prote...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1983
ISSN: 0264-6021
DOI: 10.1042/bj2120699